Nuclear sexing | Barr body | Davidson body | Sex determination | Forensic medicine
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Nuclear sexing is a method of sexing cells which may help in determining sex in doubtful cases, decomposed and mutilated bodies and fragmentary remains.
Barr body (sex chromatin): It is the condensed, inactive, single X-chromosome found in the nuclei of somatic cells of most females and whose presence is the basis of sex determination tests that are performed.
Davidson body: In females, neutrophil leucocytes contain a small nuclear attachment of drumstick form.
Quinacrine dihydrochloride is used for staining Y-chromosome that is seen as bright fluorescent body.
Fluorescent Feulgen reaction using Acriflavin Schiff reagent is used for staining X-chromosome that is seen as bright yellow spot in nuclei.
Watch the video to study in detail.
Sex determination Part 1 (Sex verification techniques)
https://youtu.be/eFnJ7eRA0Bk
Sex determination Part 2 (Nuclear sexing)
https://youtu.be/CSwae1VOMiA
Sex determination using skull and mandible
https://youtu.be/_tYoiM56wcQ
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